recombinant mouse il1b Search Results


interleukin 1β il 1β  (Bioss)
95
Bioss interleukin 1β il 1β
Interleukin 1β Il 1β, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il1b/Recombinant+mouse+IL-1b+protein%2C+C-His/pmc11992624-55-11-14
Average 95 stars, based on 1 article reviews
interleukin 1β il 1β - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
il1b recombinant mouse protein  (Thermo Fisher)
N/A
Interleukin 1 Beta (IL1B), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an
   Buy from Supplier
interleukin 1beta il 1β  (Boster Bio)
93
Boster Bio interleukin 1beta il 1β
Interleukin 1beta Il 1β, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il1b/Mouse+IL-1+beta+Recombinant+Protein/pmc06970136-62-17-22
Average 93 stars, based on 1 article reviews
interleukin 1beta il 1β - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
interleukin il 1β  (Elabscience Biotechnology)
94
Elabscience Biotechnology interleukin il 1β
Seq10 demonstrates good potential for anti-photoaging and activation of the Nrf2 signaling pathway. Seq10 demonstrates good potential for photostability and activation of the Nrf2 signaling pathway. (A) HaCaTs were treated with specified concentrations of Seq10, and cell viability was assessed using the CCK8 assay. (B) A cellular photaging model was established to evaluate the photostability potential of Seq10. (C) The effect of Seq10 treatment on the viability of HaCaTs subjected to UVA irradiation. (D) The impact of Seq10 treatment on the expression levels of MMP-1 and MMP-9 proteins within cells. (E) The effect of Seq10 treatment on the expression levels of NQO1, HO-1, and GCLM proteins within cells. (F) The influence of Seq10 treatment on the expression levels of inflammatory factors COX2 and <t>IL-1β</t> proteins within cells. (G-I) Relative mRNA expression of MMP-1, MMP-9, GCLM, HO-1, NQO1, IL-1β, and TNF-α in each group. (J) The effect of Seq10 treatment on the protein levels of Nrf2 and Keap1 within cells. (K) The expression levels of P-Nrf2 in HaCaTs whole cell lysates and Nrf2 in the nucleus were processed in Seq10. (L) Quantification of Nrf2 released from HaCaTs by qRT-PCR. (M) Immunofluorescence staining of Nrf2 protein in HaCaTs. Scale bar, 50 µm. (N) Fluorescence intensity of ROS levels. (O) Analysis of intracellular ROS levels after Nrf2 knockdown. Scale bar, 100 µm. (P) Fluorescence intensity of ROS levels. (Q) Quantification of mRNA for IL-6, IL-1β, and TNF-α secreted by HaCaTs following Nrf2 knockdown. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.
Interleukin Il 1β, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il1b/Recombinant+Mouse+IL-1+beta%2FIL1B+Protein/pmc12839140-157-0-3
Average 94 stars, based on 1 article reviews
interleukin il 1β - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
recombinant mouse il 1β  (Kingfisher Biotech)
93
Kingfisher Biotech recombinant mouse il 1β
Seq10 demonstrates good potential for anti-photoaging and activation of the Nrf2 signaling pathway. Seq10 demonstrates good potential for photostability and activation of the Nrf2 signaling pathway. (A) HaCaTs were treated with specified concentrations of Seq10, and cell viability was assessed using the CCK8 assay. (B) A cellular photaging model was established to evaluate the photostability potential of Seq10. (C) The effect of Seq10 treatment on the viability of HaCaTs subjected to UVA irradiation. (D) The impact of Seq10 treatment on the expression levels of MMP-1 and MMP-9 proteins within cells. (E) The effect of Seq10 treatment on the expression levels of NQO1, HO-1, and GCLM proteins within cells. (F) The influence of Seq10 treatment on the expression levels of inflammatory factors COX2 and <t>IL-1β</t> proteins within cells. (G-I) Relative mRNA expression of MMP-1, MMP-9, GCLM, HO-1, NQO1, IL-1β, and TNF-α in each group. (J) The effect of Seq10 treatment on the protein levels of Nrf2 and Keap1 within cells. (K) The expression levels of P-Nrf2 in HaCaTs whole cell lysates and Nrf2 in the nucleus were processed in Seq10. (L) Quantification of Nrf2 released from HaCaTs by qRT-PCR. (M) Immunofluorescence staining of Nrf2 protein in HaCaTs. Scale bar, 50 µm. (N) Fluorescence intensity of ROS levels. (O) Analysis of intracellular ROS levels after Nrf2 knockdown. Scale bar, 100 µm. (P) Fluorescence intensity of ROS levels. (Q) Quantification of mRNA for IL-6, IL-1β, and TNF-α secreted by HaCaTs following Nrf2 knockdown. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.
Recombinant Mouse Il 1β, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il1b/Mouse+IL-1+beta+Recombinant+Protein/pmc08106025-82-0-10
Average 93 stars, based on 1 article reviews
recombinant mouse il 1β - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
interleukin 1 beta il 1β levels  (Cusabio)
93
Cusabio interleukin 1 beta il 1β levels
Seq10 demonstrates good potential for anti-photoaging and activation of the Nrf2 signaling pathway. Seq10 demonstrates good potential for photostability and activation of the Nrf2 signaling pathway. (A) HaCaTs were treated with specified concentrations of Seq10, and cell viability was assessed using the CCK8 assay. (B) A cellular photaging model was established to evaluate the photostability potential of Seq10. (C) The effect of Seq10 treatment on the viability of HaCaTs subjected to UVA irradiation. (D) The impact of Seq10 treatment on the expression levels of MMP-1 and MMP-9 proteins within cells. (E) The effect of Seq10 treatment on the expression levels of NQO1, HO-1, and GCLM proteins within cells. (F) The influence of Seq10 treatment on the expression levels of inflammatory factors COX2 and <t>IL-1β</t> proteins within cells. (G-I) Relative mRNA expression of MMP-1, MMP-9, GCLM, HO-1, NQO1, IL-1β, and TNF-α in each group. (J) The effect of Seq10 treatment on the protein levels of Nrf2 and Keap1 within cells. (K) The expression levels of P-Nrf2 in HaCaTs whole cell lysates and Nrf2 in the nucleus were processed in Seq10. (L) Quantification of Nrf2 released from HaCaTs by qRT-PCR. (M) Immunofluorescence staining of Nrf2 protein in HaCaTs. Scale bar, 50 µm. (N) Fluorescence intensity of ROS levels. (O) Analysis of intracellular ROS levels after Nrf2 knockdown. Scale bar, 100 µm. (P) Fluorescence intensity of ROS levels. (Q) Quantification of mRNA for IL-6, IL-1β, and TNF-α secreted by HaCaTs following Nrf2 knockdown. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.
Interleukin 1 Beta Il 1β Levels, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il1b/Recombinant+Mouse+Interleukin-1+beta(Il1b)%2Cpartial/pm25723390-73-10-22
Average 93 stars, based on 1 article reviews
interleukin 1 beta il 1β levels - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
il1b nm 008361 mouse recombinant protein  (OriGene)
N/A
Recombinant protein of mouse Interleukin 1B IL 1B
   Buy from Supplier
recombinant mouse il1b (118-269aa), met tagged  (Creative BioMart)
N/A
Recombinant Mouse IL1B (Val118 ­ Ser269, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.IL­1 is a name that designates two pleiotropic cytokines, IL­1μ (IL­1F1) and
   Buy from Supplier
mouse interleukin 1 beta (il-1b) recombinant protein  (Thermo Fisher)
N/A
Mouse Interleukin 1 beta (IL-1b) Recombinant Protein for Western Blot, IP, ELISA, Ctrl
   Buy from Supplier
recombinant mouse il1b  (Creative BioMart)
N/A
Recombinant Mouse Il1b was expressed in CHO Cells.Interleukin-1 (IL-1) is a family of cytokines that play a central role in the regulation of immune and inflammatory responses to infections or sterile insults. IL-1α and IL-1β
   Buy from Supplier
recombinant mouse il1b protein  (Creative BioMart)
N/A
Recombinant Mouse IL1B full length or partial length protein was expressed.http://www.creativebiomart.net/Recombinant-Mouse-IL1B-Protein-442905.htm
   Buy from Supplier

Image Search Results


Seq10 demonstrates good potential for anti-photoaging and activation of the Nrf2 signaling pathway. Seq10 demonstrates good potential for photostability and activation of the Nrf2 signaling pathway. (A) HaCaTs were treated with specified concentrations of Seq10, and cell viability was assessed using the CCK8 assay. (B) A cellular photaging model was established to evaluate the photostability potential of Seq10. (C) The effect of Seq10 treatment on the viability of HaCaTs subjected to UVA irradiation. (D) The impact of Seq10 treatment on the expression levels of MMP-1 and MMP-9 proteins within cells. (E) The effect of Seq10 treatment on the expression levels of NQO1, HO-1, and GCLM proteins within cells. (F) The influence of Seq10 treatment on the expression levels of inflammatory factors COX2 and IL-1β proteins within cells. (G-I) Relative mRNA expression of MMP-1, MMP-9, GCLM, HO-1, NQO1, IL-1β, and TNF-α in each group. (J) The effect of Seq10 treatment on the protein levels of Nrf2 and Keap1 within cells. (K) The expression levels of P-Nrf2 in HaCaTs whole cell lysates and Nrf2 in the nucleus were processed in Seq10. (L) Quantification of Nrf2 released from HaCaTs by qRT-PCR. (M) Immunofluorescence staining of Nrf2 protein in HaCaTs. Scale bar, 50 µm. (N) Fluorescence intensity of ROS levels. (O) Analysis of intracellular ROS levels after Nrf2 knockdown. Scale bar, 100 µm. (P) Fluorescence intensity of ROS levels. (Q) Quantification of mRNA for IL-6, IL-1β, and TNF-α secreted by HaCaTs following Nrf2 knockdown. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Journal: International Journal of Biological Sciences

Article Title: Novel GS5 sericin mitigates UVA-induced photoaging by activating Nrf2 and inhibiting the JAK-STAT pathway

doi: 10.7150/ijbs.123702

Figure Lengend Snippet: Seq10 demonstrates good potential for anti-photoaging and activation of the Nrf2 signaling pathway. Seq10 demonstrates good potential for photostability and activation of the Nrf2 signaling pathway. (A) HaCaTs were treated with specified concentrations of Seq10, and cell viability was assessed using the CCK8 assay. (B) A cellular photaging model was established to evaluate the photostability potential of Seq10. (C) The effect of Seq10 treatment on the viability of HaCaTs subjected to UVA irradiation. (D) The impact of Seq10 treatment on the expression levels of MMP-1 and MMP-9 proteins within cells. (E) The effect of Seq10 treatment on the expression levels of NQO1, HO-1, and GCLM proteins within cells. (F) The influence of Seq10 treatment on the expression levels of inflammatory factors COX2 and IL-1β proteins within cells. (G-I) Relative mRNA expression of MMP-1, MMP-9, GCLM, HO-1, NQO1, IL-1β, and TNF-α in each group. (J) The effect of Seq10 treatment on the protein levels of Nrf2 and Keap1 within cells. (K) The expression levels of P-Nrf2 in HaCaTs whole cell lysates and Nrf2 in the nucleus were processed in Seq10. (L) Quantification of Nrf2 released from HaCaTs by qRT-PCR. (M) Immunofluorescence staining of Nrf2 protein in HaCaTs. Scale bar, 50 µm. (N) Fluorescence intensity of ROS levels. (O) Analysis of intracellular ROS levels after Nrf2 knockdown. Scale bar, 100 µm. (P) Fluorescence intensity of ROS levels. (Q) Quantification of mRNA for IL-6, IL-1β, and TNF-α secreted by HaCaTs following Nrf2 knockdown. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Article Snippet: Interleukin (IL)-1β (PDEM100209, Elabscience, Wuhan, Hubei, China) and IL-6 (E-EL-M0044, Elabscience, Wuhan, Hubei, China) levels were quantified by enzyme linked immunosorbent assay (ELISA) kits per manufacturer's protocol.

Techniques: Activation Assay, CCK-8 Assay, Irradiation, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining, Fluorescence, Knockdown, Standard Deviation

GS5 sericin protects keratinocytes from UVA-induced cellular senescence in vitro . (A) Effects of WT or GS5 sericin treatment on the viability of HaCaTs after UVA irradiation. (B) EdU (red) proliferation assay in HaCaTs. Hoechst (blue) staining for cell nuclei. Scale bar, 100 µm. (C) Proportion of EdU-labeled mitotic cells. (D) Representative images of SA-β-gal staining in HaCaTs. Scale bar, 50 μm. (E) Quantification of SA-β-gal positive cells. (F) Wound healing assay to determine the migratory capacity of HaCaTs. Scale bar, 400 μm. (G) Quantification of HaCaTs cell migration assay. (H) Representative immunofluorescence staining images of ROS (green) positive cells. Hoechst (blue) staining for cell nuclei. Scale bar, 100 μm. (I) Fluorescence intensity of ROS levels. (J) Representative immunofluorescence staining images of γ-H2Ax (green) positive cells. DAPI (blue) staining for cell nuclei. Scale bar, 50 μm. (K) Quantification of the average number of γ-H2Ax foci per cell. (L) Western blot analysis showing the change of IL-6 and iNOS in HaCaTs. (M) Quantification of IL-6, IL-1β, and TNF-α released by HaCaTs detected by qRT-PCR. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Journal: International Journal of Biological Sciences

Article Title: Novel GS5 sericin mitigates UVA-induced photoaging by activating Nrf2 and inhibiting the JAK-STAT pathway

doi: 10.7150/ijbs.123702

Figure Lengend Snippet: GS5 sericin protects keratinocytes from UVA-induced cellular senescence in vitro . (A) Effects of WT or GS5 sericin treatment on the viability of HaCaTs after UVA irradiation. (B) EdU (red) proliferation assay in HaCaTs. Hoechst (blue) staining for cell nuclei. Scale bar, 100 µm. (C) Proportion of EdU-labeled mitotic cells. (D) Representative images of SA-β-gal staining in HaCaTs. Scale bar, 50 μm. (E) Quantification of SA-β-gal positive cells. (F) Wound healing assay to determine the migratory capacity of HaCaTs. Scale bar, 400 μm. (G) Quantification of HaCaTs cell migration assay. (H) Representative immunofluorescence staining images of ROS (green) positive cells. Hoechst (blue) staining for cell nuclei. Scale bar, 100 μm. (I) Fluorescence intensity of ROS levels. (J) Representative immunofluorescence staining images of γ-H2Ax (green) positive cells. DAPI (blue) staining for cell nuclei. Scale bar, 50 μm. (K) Quantification of the average number of γ-H2Ax foci per cell. (L) Western blot analysis showing the change of IL-6 and iNOS in HaCaTs. (M) Quantification of IL-6, IL-1β, and TNF-α released by HaCaTs detected by qRT-PCR. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Article Snippet: Interleukin (IL)-1β (PDEM100209, Elabscience, Wuhan, Hubei, China) and IL-6 (E-EL-M0044, Elabscience, Wuhan, Hubei, China) levels were quantified by enzyme linked immunosorbent assay (ELISA) kits per manufacturer's protocol.

Techniques: In Vitro, Irradiation, Proliferation Assay, Staining, Labeling, Wound Healing Assay, Cell Migration Assay, Immunofluorescence, Fluorescence, Western Blot, Quantitative RT-PCR, Standard Deviation

Nrf2 is essential for the anti-photoaging effect and SASP of GS5. (A) Western blot analysis for Nrf2 and P-Nrf2 expression levels in whole cell lysates. (B) Western blot analysis for Nrf2 expression levels in the nucleus. (C - D) Relative mRNA expression of Nrf2, GCLM, HO-1, and NQO1 in the control group and UVA radiation group. (E) Immunofluorescence images of Nrf2 (green) at 12 h and 24 h in each group. Scale bar, 50 μm. (F - G) Changes in relative mRNA levels of Nrf2, GCLM, HO-1, and NQO1 in irradiated HaCaTs treated with GS5 over time. (H) Quantification of mRNA secretion of IL-6, IL-1β, TNF-α, MMP-1, and MMP-9 in HaCaTs after Nrf2 knockdown. (I) Analysis of ROS (green) levels in GS5-treated cells after Nrf2 knockdown. Scale bar, 100 µm. (J) Fluorescence intensity of ROS levels. (K) Representative images of SA-β-gal staining in HaCaTs after Nrf2 knockdown. Scale bar, 50 μm. (L) Quantification of SA-β-gal positive cells. (M) Representative images of cell colonies formed in the survival assay after Nrf2 knockdown. (N) Quantification of cell colony formation. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Journal: International Journal of Biological Sciences

Article Title: Novel GS5 sericin mitigates UVA-induced photoaging by activating Nrf2 and inhibiting the JAK-STAT pathway

doi: 10.7150/ijbs.123702

Figure Lengend Snippet: Nrf2 is essential for the anti-photoaging effect and SASP of GS5. (A) Western blot analysis for Nrf2 and P-Nrf2 expression levels in whole cell lysates. (B) Western blot analysis for Nrf2 expression levels in the nucleus. (C - D) Relative mRNA expression of Nrf2, GCLM, HO-1, and NQO1 in the control group and UVA radiation group. (E) Immunofluorescence images of Nrf2 (green) at 12 h and 24 h in each group. Scale bar, 50 μm. (F - G) Changes in relative mRNA levels of Nrf2, GCLM, HO-1, and NQO1 in irradiated HaCaTs treated with GS5 over time. (H) Quantification of mRNA secretion of IL-6, IL-1β, TNF-α, MMP-1, and MMP-9 in HaCaTs after Nrf2 knockdown. (I) Analysis of ROS (green) levels in GS5-treated cells after Nrf2 knockdown. Scale bar, 100 µm. (J) Fluorescence intensity of ROS levels. (K) Representative images of SA-β-gal staining in HaCaTs after Nrf2 knockdown. Scale bar, 50 μm. (L) Quantification of SA-β-gal positive cells. (M) Representative images of cell colonies formed in the survival assay after Nrf2 knockdown. (N) Quantification of cell colony formation. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Article Snippet: Interleukin (IL)-1β (PDEM100209, Elabscience, Wuhan, Hubei, China) and IL-6 (E-EL-M0044, Elabscience, Wuhan, Hubei, China) levels were quantified by enzyme linked immunosorbent assay (ELISA) kits per manufacturer's protocol.

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Irradiation, Knockdown, Fluorescence, Staining, Clonogenic Cell Survival Assay, Standard Deviation

GS5 sericin alleviates UVA-induced skin photodamage in mice. (A) Immunohistochemical staining of COL1A2, IL-6, and MMP-1 in the dorsal skin of mice. Scale bar, 50 μm. (B) Quantification of COL1A2 by qRT-PCR. (C) Quantification of IL-6 by qRT-PCR. (D) Quantification of MMP-1. (E) Immunofluorescence staining of Lamin B (red) in the dorsal skin of mice. Scale bar, 100 μm. (F) Quantification of Lamin B positive cells. (G) Quantification of CAT, MDA, and SOD in the skin. (H) Quantification of IL-6 and IL-1β released from the skin measured by ELISA. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Journal: International Journal of Biological Sciences

Article Title: Novel GS5 sericin mitigates UVA-induced photoaging by activating Nrf2 and inhibiting the JAK-STAT pathway

doi: 10.7150/ijbs.123702

Figure Lengend Snippet: GS5 sericin alleviates UVA-induced skin photodamage in mice. (A) Immunohistochemical staining of COL1A2, IL-6, and MMP-1 in the dorsal skin of mice. Scale bar, 50 μm. (B) Quantification of COL1A2 by qRT-PCR. (C) Quantification of IL-6 by qRT-PCR. (D) Quantification of MMP-1. (E) Immunofluorescence staining of Lamin B (red) in the dorsal skin of mice. Scale bar, 100 μm. (F) Quantification of Lamin B positive cells. (G) Quantification of CAT, MDA, and SOD in the skin. (H) Quantification of IL-6 and IL-1β released from the skin measured by ELISA. All data are presented as means ± standard deviation (SD) (n = 3). Statistical significance is denoted by * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. UVA-irradiated group.

Article Snippet: Interleukin (IL)-1β (PDEM100209, Elabscience, Wuhan, Hubei, China) and IL-6 (E-EL-M0044, Elabscience, Wuhan, Hubei, China) levels were quantified by enzyme linked immunosorbent assay (ELISA) kits per manufacturer's protocol.

Techniques: Immunohistochemical staining, Staining, Quantitative RT-PCR, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Standard Deviation, Irradiation